Development of duplex PCR for differential detection of goatpox and sheeppox viruses
نویسندگان
چکیده
منابع مشابه
Development of duplex PCR for differential detection of goatpox and sheeppox viruses
BACKGROUND Clinically, sheeppox and goatpox have the same symptoms and cannot be distinguished serologically. A cheaper and easy method for differential diagnosis is important in control of this disease in endemic region. METHODS A duplex PCR assay was developed for the specific differential detection of Goatpox virus (GTPV) and Sheeppox virus (SPPV), using two sets of primers based on viral ...
متن کاملThe genomes of sheeppox and goatpox viruses.
Sheeppox virus (SPPV) and goatpox virus (GTPV), members of the Capripoxvirus genus of the Poxviridae, are etiologic agents of important diseases of sheep and goats in northern and central Africa, southwest and central Asia, and the Indian subcontinent. Here we report the genomic sequence and comparative analysis of five SPPV and GTPV isolates, including three pathogenic field isolates and two a...
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Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...
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a simple, rapid and low-cost scanner spectroscopy method for the glucose determination by utilizing glucose oxidase and cdte/tga quantum dots as chromoionophore has been described. the detection was based on the combination of the glucose enzymatic reaction and the quenching effect of h2o2 on the cdte quantum dots (qds) photoluminescence.in this study glucose was determined by utilizing glucose...
Detection and differentiation of sheeppox virus and goatpox virus from clinical samples
Aim: To detect and differentiate Capripox virus (sheeppox virus and goatpox virus) infections by using 30 kDa RNA polymerase subunit (RPO30) gene based PCR. Materials and Methods: Two capripox viruses' viz., sheep pox virus (SPPV) and goatpox virus (GTPV) from clinical samples of different outbreaks were detected and differentiated using capri pox virus (CaPVs) genotyping PCR targeting the CaPV...
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ژورنال
عنوان ژورنال: BMC Veterinary Research
سال: 2017
ISSN: 1746-6148
DOI: 10.1186/s12917-017-1179-0